How have the results been disseminated to communities of interest?Through peer-reviewed jounral publications and conference proceedings.
#PROTEIN SCAFFOLD MYCOBACTERIUM AVIUM PARATUBERCULOSIS PROFESSIONAL#
What opportunities for training and professional development has the project provided? Progress 03/01/15 to 02/29/16 Outputs Target Audience: Validation of candidate antigens and development of immunoassays for the sensitive and specific detection of MAP infected animals. Characterization of the MAP immunoproteome and dynamics of the humoral response to MAP proteins using protein microarrays and,Specific Aim 2. To test this hypothesis, we will employ powerful high-throughput protein microarray based approaches and well characterized banks of serum samples from animals at different stages of JD to identify combinations of proteins for the improved detection of MAP infection with high levels of sensitivity and specificity.Our Studies will be carried out with two Specific Aims:Specific Aim 1. Goals / Objectives We propose to test the overall hypothesis that comprehensive analyses of the immunoproteome and dynamical immune response to infection will enable the identification of key antigens for the early detection of MAP infected animals and provide a rationale framework for the development of the next generation of immunodiagnostic assays. Together, the knowledge and resources gained from our investigations will enable the construction of a rational framework for the development and validation of future generations of diagnostic assays to enhance US animal health and productivity through better control of JD. These markers will then be evaluated for the ability to sensitively and specifically identify MAP infected animals early during the infection process. To test this hypothesis, we will apply powerful state-of-the-art molecular approaches to screen well-characterized sets of serum samples from cattle and goats at different stages of JD for potential early markers of infection. Our preliminary studies support the hypothesis that some MAP proteins may serve as early markers of infection. Progress in controlling the spread of infection has been impeded by the lack of reliable diagnostic tests that can identify animals early during the infection process. Non Technical Summary Mycobacterium aviumsubspeciesparatuberculosis(MAP) causes Johne's disease (JD) in cattle, sheep, and goat, and results in considerable production and economic losses to US animal agriculture.
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